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. 2014 Feb 25;15:159. doi: 10.1186/1471-2164-15-159

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Copyright © 2014 Krishnan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The erg1 mRNA increases its association with polysomes during ER stress. Mycelial extracts from control (untreated) and TM-treated cultures were fractionated into 7 pools. The RNA in each pool was then separated by RNA gel electrophoresis and the level of erg1 mRNA in each fraction was determined by hybridization to an erg1 probe. Band intensities were quantified by phosphorimager analysis and shown on the top graph. A representative OD₂₅₄ profile is superimposed on the graph for reference. The findings demonstrate increased erg1 mRNA levels in the polysome fraction during ER stress.