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. 2014 Feb 22;14:118. doi: 10.1186/1471-2407-14-118

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Copyright © 2014 Pinto et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Intracellular expression of chemokines. Representative examples for the quantification of intracellular chemokine expression by both flow cytometry (A, B) and immunocytochemistry (C, D) are shown. Mean fluorescence indexes and overlaid histograms of PE fluorescence of specific anti-receptor monoclonal antibody (continuous red line) and correspondent isotypic control (discontinuous black line) are shown for CXCL12 in the WM-115 cell line (A) and for CCL27 in the WM-266.4 cell line (B). Corresponding immunocytochemical staining of CXCL12 in WM-115 (C) and CCL27 in WM-266.4 (D).