Table 7.
Intracellular chemokine expression after xenotransplantation
| |
CXCL9
|
CXCL10
|
CXCL11
|
CXCL12
|
CCL19
|
CCL21
|
CCL27
|
CCL28
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % | MFI | % | MFI | % | MFI | % | MFI | % | MFI | % | MFI | % | MFI | % | MFI | |
| WM-115 |
28.31 |
2.06 |
0.46 |
1.12 |
2.66 |
1.24 |
81.77 |
3.96 |
98.79 |
20.00 |
15.55 |
1.65 |
94.06 |
4.89 |
ns |
0.94 |
| WM-115-X |
ns |
1.10 |
ns |
0.90 |
ns |
0.96 |
0.30 |
0.94 |
50.38 |
3.80 |
ns |
1.05 |
22.39 |
1.62 |
ns |
0.97 |
| WM-115-CX |
50.39 |
5.36 |
4.33 |
2.54 |
15.24 |
5.25 |
79.83 |
5.08 |
98.64 |
36.76 |
14.46 |
4.24 |
98.17 |
13.36 |
3.51 |
2.98 |
| WM-266 |
71.84 |
4.57 |
15.19 |
1.74 |
46.26 |
3.24 |
98.24 |
9.56 |
98.58 |
30.17 |
60.21 |
3.71 |
98.50 |
11.38 |
1.14 |
1.25 |
| WM-266-X |
2.12 |
1.46 |
2.53 |
1.28 |
22.73 |
1.93 |
3.81 |
1.42 |
81.95 |
10.43 |
2.38 |
1.33 |
54.41 |
3.39 |
1.25 |
1.19 |
| WM-266-CX | 67.84 | 7.02 | 3.72 | 1.41 | 37.75 | 4.98 | 95.52 | 14.66 | 98.10 | 43.82 | 50.06 | 4.00 | 97.12 | 12.36 | 0.29 | 0.97 |
Mean percentage (%) and mean fluorescence index (MFI; calculated as the ratio between the mean fluorescence of samples stained with the mAb anti-receptor and correspondent isotypic control) of cells which significantly expressed (p < 0.05) chemokine ligands intracellularly. WM-115 and WM-266 represent the mean values of the original cell lines; WM-115-X and WM-266-X represent the mean values of the different xenografts; WM-115-CX and WM-266-CX represent the mean values of the different cell lines obtained after culturing the xenografts.