Table 2.
Mouse plasma and tissue sterol analyses
| Plasma (mg/dL) | ||||
| Group | Cholesterol | Campesterol | Stigmasterol | β-Sitosterol |
| Abcg8+/+ | 73.0 ± 14 | 1.1 ± 0.7 | ND | 0.5 ± 0.5 |
| Abcg8+/- | 54.0 ± 10* | 1.8 ± 0.3* | ND | 0.8 ± 0.2* |
| Abcg8-/- | 35.0 ± 6.0* | 8.8 ± 2.1* | ND | 18.5 ± 5.0* |
| Liver (μg/g wet tissue) | ||||
| Group | Cholesterol | Campesterol | Stigmasterol | β-Sitosterol |
| Abcg8+/+ | 2280 ± 310 | 52.2 ± 15.0 | ND | 16.7 ± 8.6 |
| Abcg8+/- | 2460 ± 588 | 76.4 ± 12.3* | 1.6 ± 3.3* | 26.7 ± 6.4* |
| Abcg8-/- | 1202 ± 264* | 233.9 ± 48.1* | 22.7 ± 6.3* | 376.3 ± 91.6* |
| Spleen (μg/g wet tissue) | ||||
| Group | Cholesterol | Campesterol | Stigmasterol | β-Sitosterol |
| Abcg8+/+ | 2702 ± 310 | 41.7 ± 7.6 | 15.1 ± 14.1 | 21.9 ± 10.9 |
| Abcg8+/- | 2578 ± 320 | 66.8 ± 18.8* | 14.5 ± 16.9* | 32.2 ± 6.1* |
| Abcg8-/- | 1779 ± 195* | 353.8 ± 97.5* | ND* | 436.6 ± 148.5* |
| Brain (μg/g wet tissue) | ||||
| Group | Cholesterol | Plant sterols | Desmosterol | Lathosterol |
| Abcg8+/+ | 11301 ± 156 | 10.2 ± 3.9 | 102.0 ± 13.3 | 52.0 ± 0.3 |
| Abcg8+/- | 11846 ± 814 | 24.5 ± 5.5 | 113.7 ± 25.5 | 49.1 ± 4.2 |
| Abcg8-/- | 11846 ± 1061 | 63.0 ± 6.5 | 123 ± 18.3 | 56.9 ± 9.5 |
Animals used in these studies were 12 weeks of age, a mixture of male and female and fed a regular chow diet. *P < 0.05 for -/- versus +/+ and -/- versus +/-. ND, not detectable.