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. 2013 Nov 13;23(7):1869–1878. doi: 10.1093/hmg/ddt579

Figure 3.

Figure 3.

Hmox1 overexpression is sufficient for the observed muscle improvement. (A) RT-PCR confirmation of Hmox1 overexpression in sapje fish and sapje-like fish (*sapje, P = 9.09e-13; **sapje-like, P = 1.57e-04; t-test, n = 3). (B) Injecting 50 pg of zebrafish hmox1 mRNA into eggs from a heterozygous mating decreases the fraction of affected offspring (*sapje, P = 3.48e-05; **sapje-like, P = 1.0e-04; t-test, n = 3). (C) Measurement of the amount of cGMP in hmox1-overexpressed sapje fish and sapje-like fish (*sapje, P = 0.0078; **sapje-like, P = 0.018; t-test, n = 3). (D) Normal skeletal muscle structure in dystrophin-null sapje fish injected with 50 pg hmox1. Restored dystrophin-null fish have normal muscle structure similar to those of wild type. Dark midlines reflect pigmentation.