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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Sep 10;1838(4):1143–1152. doi: 10.1016/j.bbamem.2013.08.020

Figure 4. Mutations in FIV Env confer resistance to TSG-5’.

Figure 4

A) The two mutations in FIV Env that were detected by sequencing cDNA from infected T5’hi cells are shown to scale with functional domains labeled. gp95 is the surface subunit, analogous to HIV-1 gp120. gp36 is the transmembrane subunit analogous to HIV-1 gp41, which contains the two heptad repeats (HR) involved in membrane fusion, the transmembrane domain (TMD), and the cytoplasmic tail (CT). B) Mutations in FIV Env that putatively conferred resistance to TSG-5’ were introduced into the WT molecular FIV clone by site-directed mutagenesis. Mutant clones were then tested against WT FIV for replication capacity in transfected CRFK-zeo and T5’hi cells.