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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Sep 10;1838(4):1143–1152. doi: 10.1016/j.bbamem.2013.08.020

Figure 7. TSG-5’ resistance mutations enhance cell-cell FIV transmission.

Figure 7

CRFK-zeo cells chronically infected with either WT FIV or one of the TSG-5’-resistant mutants were counted and mixed with uninfected CRFK-zeo or T5’hi cells in suspension at a final ratio of 1:20 (5% Gag-positive cells). Cell mixtures were aliquoted in cell culture plates and incubated overnight. Adherent cells were washed to remove any released cell-free virions and covered with melted growth medium containing 0.5% high-melting agarose, then incubated at 37°C. For each time interval, overlays were removed and the cell monolayer was resuspended, washed, and fixed. FIV p24 was later detected in all fixed samples by immunofluorescence and flow cytometry. Cells from the initial coculture were also fixed and analyzed to verify the baseline ratio of infected cells.