Table 1.

Calibration Linearity in Plasma (A) or Plasma Pre-Stabilized with 0.1% HCl (B)

A
Nominal (ng/mL)	GM (ng/mL)	SD (ng/mL)	DEV (%)	CV (%)	n
1	1.00	0.06	−0.09	5.97	8
5	4.88	0.23	−2.40	4.71	8
10	10.18	0.40	1.76	3.96	8
50	50.57	1.99	1.14	3.94	8
100	102.90	3.69	2.90	3.58	8
250	249.33	12.61	−0.27	5.06	8
500	479.79	34.27	−4.04	7.14	8

B
Nominal (ng/mL)	GM (ng/mL)	SD (ng/mL)	DEV (%)	CV (%)	n
1	0.99	0.07	−0.63	7.28	8
5	5.01	0.64	0.23	12.74	8
10	10.40	1.35	4.04	12.98	8
50	50.27	3.66	0.55	7.27	8
100	99.64	7.42	−0.36	7.45	8
250	249.02	23.76	−0.39	9.54	8
500	481.33	37.44	−3.73	7.78	8

Abbreviations: GM, grand mean; SD, standard deviation; DEV (%), relative deviation from nominal value; CV (%), coefficient of variation; n, number of replicate observations within each validation run, i.e. two samples at each concentration were run on four separate occasions, for a total (n) of eight samples at each concentration.