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. 2013 Dec 3;8(2):285–296. doi: 10.1016/j.molonc.2013.11.006

Figure 3.

Figure 3

The C‐Jun/AP‐1 pathway mediated SUMOylated RhoGDIα‐repressed cyclin d1 transcription and expression, as well as cell cycle regulation. (A) the RhoGDIα‐K138R cells stably transfected with either TAM67 construct or the control empty vector were cultured in 2% FBS medium for 24 h and the cell extracts were subjected to Western blot. (B) the RhoGDIα‐K138R stable transfectants were established by co‐transfection of cyclin d1 promoter luciferase reporter with TAM67 and blasticidin selection. After synchronization, cells were cultured in 2% FBS medium for 24 h and the cells were then extracted for determination of luciferase activity. The symbol (*) indicates a significant repression in comparison to that of the control vector transfectant (p < 0.05); (C) the RhoGDIα‐K138R cells' stable transfectants were established by transfection of ‐963 CD1 Luc and ‐963 AP‐1mut CD1 Luc and blasticidin selection. After synchronization, cells were cultured in 2% FBS medium for 24 h, and the cells were then extracted for determination of luciferase activity. The symbol (*) indicates a significant repression as compared with that of ‐963 CD1 Luc reporter transfectant (p < 0.05); (D) the cell cycle profile in the indicated cell transfectants was determined by flow cytometry analysis.