Figure 4.

Line plots showing that glycerol is elevated more than 240 fold in blood plasma, and more than 30 to 60 fold in tissues at one and six hours after glycerol administration (data ± SEM). Glycerol concentrations are plotted on a logarithmic scale for plasma (filled triangle), hippocampus (light gray square), cerebellum (dark gray circle) and liver (unfilled triangle). Newborn rabbits, untreated (time 0) or glycerol treated (6.5 g/kg i.p.), were killed at one or six hours post treatment and samples of brain and liver tissue were collected and snap frozen, then homogenized and assayed for glycerol content by ELISA (N = 3 per time point). Subarachnoid hemorrhages were evident shortly after injection, but no intraventricular hemorrhages were detected after brain removal.