Table 1.

PCR thermal cycling parameters.

Step		Temperature/duration
		1st PCR	2nd PCR	HBV genotyping	rs1082	rs819	rs592
No. of amplification cycles		35	35	35	33	33	33
Initial inactivation/denaturation		95°C/10 min	95°C/10 min	95°C/10 min	95°C/3 min	95°C/3 min	95°C/3 min
Parameters of amplification	Denaturation	94°C/30 sec	94°C/30 sec	94°C/30 sec	95°C/30 sec	95°C/30 sec	95°C/30 sec
	Annealing	56°C/30sec	57°C/30 sec	57°C/30 sec	57.9°C/30 sec	61.4°C/30 sec	53.7°C/30 sec
	Extension	72°C/30 sec	72°C/30 sec	72°C/90 sec	72°C/60 sec	72°C/60 sec	72°C/60 sec
Final extension		72°C/10 min	72°C/10 min	72°C/10 min	72°C/1 min	72°C/1 min	72°C/1 min
Hold		4°C	4°C	4°C	4°C	4°C	4°C