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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Acquir Immune Defic Syndr. 2014 Apr 1;65(4):405–413. doi: 10.1097/QAI.0000000000000047

Figure 2. Infiltrate staining and quantification of cervico-vaginal tissue taken from SIVsmB7 and CEM mock-control-treated animals.

Figure 2

(A – Endocervix) [Top Panel: CD123 Staining 20x Image] SIVsmB7 treatment (left) induced large-scale pDC infiltration with cells directly underneath or near the columnar epithelium as compared to mock control (p=0.0317) [2nd panel: Mx1 Staining 20x Image] Corresponding with increased pDC infiltration in SIVsmB7 treated macaques there was increased levels of Interferon Responsive Gene product (ISG) Mx1 in treated macaques compared to controls [3rd panel: CD4 Staining 20x Image] SIVsmB7 treatment (left) induced moderate infiltration of CD4+ T-Cells as compared to CEM mock treatment (p=0.0357) [Bottom panel: CD68 Staining 20x Image] SIVsmB7 treatment (left) did not induce a significant increase in macrophage infiltration as compared to CEM mock control (right), but visually, macrophages appeared closer to and, in some cases, permeated the columnar epithelium, suggesting a trend of increased infiltration (p=0.0952) (B – Vagina) [Top panel: CD123 Staining 20x Image] SIVsmB7 treatment (left) induced no noticeable level of pDC infiltration as compared to CEM mock-control-treated animals (bottom) (p=0.2000) [Middle panel: CD4 Staining 20x Image] SIVsmB7 treatment (left) induced a sizable increase in CD4 T-Cell staining, with many positive staining cells found directly underneath the striated vaginal epithelium and comparably fewer cells found in the vaginal mucosa of CEM mock-control-treated animals (right) (p=0.0476) [Bottom panel: CD68 Staining 20x Image] Macaques had moderate levels of macrophage staining within the vaginal mucosa regardless of treatment. This level of CD68 staining was not impacted by treatment (p=0.3524). Pair-wise comparisons were done with Wilcoxon Rank-Sum tests.