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. 2014 Mar 5;9(3):e89772. doi: 10.1371/journal.pone.0089772

Figure 2. Rosemary extract modulates cell growth and induces cell cycle arrest in prostate cancer cell lines.

Figure 2

A) 22Rv1 and LNCaP cells were treated with 0 and 50 µg/ml of Rosemary Extract standardized to 40% carnosic acid (RE-40) for 24 hrs. Cell proliferation was assessed by BrdU assay. B) Cell viability of 22Rv1 and LNCaP cells was determined using MTT assay. Cells were treated with increasing concentrations of RE-40 (0–70 µM) for 48 hrs. C) Effect of RE-40 treatment on cell cycle arrest in 22Rv1 and LNCaP cells. Cells were treated with RE-40 at 50 µg/ml for 24 hrs followed by labeling using the APODIRECT kit for flow cytometric analysis. Columns, results are representative of experiments performed in minimum of three replicates; bars, SD. *P<0.05, **P<0.01, ***P<0.001, RE-40 treated samples versus controls.