Table 1. Summary of the mutation of the founder rats.

		Mutant rats/Total rats (%)				Bi-allelic mutant rats/Total rats (%)
	Total	ApoE	B2m	Prf1	Prkdc	ApoE	B2m	Prf1	Prkdc
Single sgRNA/Gene	15/15 (100%)	11/15 (73.3%)	9/15 (60%)	4/15 (26.7%)	10/15 (66.7%)	10/15 (66.7%)	6/15 (40%)	1/15 (6.7%)	5/15 (33.3%)
Dual sgRNAs/Gene	24/25 (96%)	19/25 (76%)	14/25 (56%)	13/25 (52%)	13/25 (52%)	15/25 (60%)	11/25 (44%)	10/25 (40%)	9/25 (36%)

Single sgRNA/Gene: A mixture of 4 sgRNAs, each targeting a single site in each of the 4 genes. Dual sgRNAs/Gene: Another distinct sgRNA of each gene, together with the tested 4 sgRNAs targeting double site in each of the 4 genes.