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. Author manuscript; available in PMC: 2014 Nov 4.
Published in final edited form as: Angew Chem Int Ed Engl. 2013 Sep 13;52(45):11852–11856. doi: 10.1002/anie.201305667

Scheme 1.

Scheme 1

Sequential one-pot multienzyme (OPME) chemoenzymatic synthesis of heparan sulfate (HS) oligosaccharide analogs. GlyK, glycokinase; NucT, monosaccharide-1-phosphate nucleotidyltransferase; PpA, inorganic pyrophosphatase; GlyT, glycosyltransferase.