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One-pot three-enzyme GlcA activation and transfer system for the synthesis of GlcAβ1–4GlcNAcα1–4GlcAβ2AA trisaccharide and derivatives. EcGalU, Escherichia coli K-12 glucose-1-phosphate uridylyltransferase; PmUgd, Pasteurella multocida strain P-1059 UDP-glucose dehydrogenase.
