Skip to main content
. 2014 Mar 5;9(3):e89841. doi: 10.1371/journal.pone.0089841

Figure 5. Western blot analysis showing the effects of doxycycline on SDF-1α-induced AKT/ERK phosphorylation in SKOV3 and SKOV3/DDP cell lines.

Figure 5

After treatment of SDF-1α (50 ng/ml) for 0, 2, 5, 10, 20, 40 minutes, AKT (A) and ERK (B) phosphorylation in SKOV3 cells were augmented. When treated SKOV3/DDP with the same dose of SDF-1α for 0, 0.5, 1, 2, 5, 10 minutes, AKT phosphorylation (C) did not change, while ERK phosphorylation (D) was augmented significantly. Then, cells were pretreated with 0, 5, 10 µg/ml doxycycline for 3 h, then treated with SDF-1α (50 ng/ml) for 10 min (SKOV3) or 1 min (SKOV3/DDP), SDF-1α-induced AKT (E) and ERK (F) phosphorylation in SKOV3 cells were only inhibited when co-application with doxycycline at dose 10 µg/ml. In SKOV3/DDP cells, SDF-1α did not induce AKT phosphorylation (G) and enhanced ERK phosphorylation (H) could not be blocked by doxycycline at dose 5 or 10 µg/ml. n = 3. *, p<0.05, **, p<0.01, SDF-1α-treated groups vs control group. #, p<0.01, SDF-1α plus doxycycline co-treated groups vs SDF-1 treated groups.