Fig. 3.

Effects of NAC on HIF-1 target gene expression in neurons in ischemic brains of rats. GLUT-3 and EPO were analyzed by double immunostaining with the neuronal marker NeuN after rats were subjected to 90 min ischemia and 24 h reperfusion. Rats received NAC (150 mg/kg, ip) at 30 min before the onset of ischemia. YC-1 (2 mg/kg, iv) was administered at 24 h and 30 min before the onset of ischemia. 2ME2 (5 mg/kg, ip) was administered at 1 h before the onset of ischemia. Selection of areas for imaging was the same as for those shown in Fig. 2. (A) Double immunostaining of GLUT-3 (green) and NeuN (red). (B) Double immunostaining of EPO (green) and NeuN (red). Scale bar, 50 μm. See supplementary material for statistical analyses on the images. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)