Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Cryobiology. 2013 Dec 9;68(1):71–78. doi: 10.1016/j.cryobiol.2013.12.001

Table 1.

Derivative solute concentrations in various dilutions of the EAFS10/10 vitrification solution

(A) (B) (C) (D) (E) (F) (G) (H) (I) (J) (K) (L) (M)
Relative
cncn.
EAFS		 Total
mass
(g)		 Total
volume
(ml)		 Molality
EG		 Molality
acetamid		 Molality
sucrose		 Molality
NaCl		 Molar
EG		 Molar
acet.		 Molar
sucrose		 Molar
NaCl		 Osmol
non-perm.		 Rel. Eq
vol cell
water
1× 11.52 10 3.23 3.27 0.72 0.15 2.283 2.283 0.503 0.105 0.996 0.277
0.75× 11.14 10 2.01 2.04 0.45 0.15 1.589 1.613 0.356 0.119 0.726 0.380
0.5× 10.76 10 1.15 1.16 0.26 0.15 0.999 1.008 0.226 0.130 0.533 0.518
0.33× 10.51 10 0.7 0.71 0.16 0.15 0.640 0.649 0.146 0.137 0.436 0.633

Columns D–G. The molalities are calculated from data in Seki and Mazur [20], Table 2.

Columns H–K: The molarities have been calculated from the molalities by the equations in an article titled “Molality” in Wikipedia, the free encyclopedia (http://en.wikipedia.org/wiki/Molality) for solutions with more than one solute. The contributions of Ficoll and BSA have been ignored. The molarity of the ith solute in moles/liter solution is Mi = Ww mi Mw, where Ww is the molecular weight of water in kg/mole (0.0018), mi is the molality of the ith solute in moles/kg of water, and Mw is the molarity of the water. Mw is equal to ρmw/(1 + Σ(mi Wi)), where ρ is the density of the solution, and mi Wi are the molalities of the ith solute times the molecular weight of that solute in kg/mole. (Note: The molarities listed in Columns H–K in Table 1 of [20] are erroneous. They were calculated incorrectly using a formula for solutions with single solutes.)

Column L: The nonpermeating solutes are sucrose and PBS (we ignore the very small contribution of the Ficoll and BSA.) Their combined osmolality is Column F + 0.276. We assume the osmolality of PBS to be equal to that of the same molality of NaCl. The osmolality of NaCl = 2φm where 2 is the number of species into which the molecule dissociates and φ is the osmotic coefficient.

Column M: The volume of water in the oocytes after equilibration with the external medium relative to the volume of water in an isotonic cell. It is 0.276/Column L.