Table 4.
Survival of mouse 8-cell embryos after freezing in EAFS10/10 with cryotop at each cooling rate and warming rate.
| Concn. EAFS |
Cooling rate (°C/min) |
Warming rate (°C/min) |
Survivals ± S.E. | % of Morphol. survivors develop to blasto. |
|
|---|---|---|---|---|---|
| Morphological observation | 8-cell to blastocysto | ||||
| 1× | 69,000 | 117,500 | 92.5 ± 7.5 (41/44, N = 8) | 92.5 ± 7.5 (41/44, N = 8) | 100 |
| 880 | 117,500 | 88.9 ± 12.2 (27/29, N = 6) | 90.0 ± 6.7 (25/30, N = 6) | 92.3 | |
| 95 | 117,500 | 64.0 ± 17.2 (16/25, N = 5) | 48.0 ± 17.5 (12/25, N = 5) | 75 | |
| 1× | 69,000 | 4,050 | 74.3 ± 16.7 (26/35, N = 7) | 65.7 ± 14.9 (23/35, N = 7) | 88.5 |
| 880 | 4,050 | 44.2 ± 17.4 (18/41, N = 8) | 17.1 ± 9.6 (7/41, N = 8) | 38.9 | |
| 95 | 4,050 | 71.1 ± 9.5 (24/34, N = 6) | 65.0 ± 9.5 (22/34, N = 6) | 91.7 | |
| 1× | 69,000 | 610 | 23.3 ± 16.4 (7/30, N = 6) | 3.3 ± 3.3 (1/30, N = 6) | 14.3 |
| 880 | 610 | 0.0 ± 0.0 (0/25, N = 5) | 0.0 ± 0.0 (0/25, N = 5) | 0 | |
| 95 | 610 | 20.0 ± 30.6 (6/30, N = 6) | 13.3 ± 18.9 (4/30, N = 6) | 66.7 | |
| 0.75× | 69,000 | 117,500 | 100.0 ± 0.0 (38/38, N = 7) | 92.4 ± 3.6 (35/38, N = 7) | 92.1 |
| 880 | 117,500 | 100.0 ± 0.0 (32/32, N = 6) | 96.7 ± 3.3 (31/32, N = 6) | 96.9 | |
| 95 | 117,500 | 88.0 ± 12.0 (22/25, N = 5) | 64.0 ± 22.3 (16/25, N = 5) | 72.8 | |
| 0.75× | 69,000 | 4,050 | 55.5 ± 8.8 (25/45, N = 9) | 33.3 ± 10.0 (15/45, N = 9) | 60 |
| 880 | 4,050 | 31.4 ± 14.8 (12/36, N = 7) | 25.7 ± 13.6 (10/36, N = 7) | 83.3 | |
| 95 | 4,050 | 59.0 ± 14.6 (23/38, N = 7) | 46.2 ± 13.0 (18/38, N = 7) | 78.3 | |
| 0.5× | 69,000 | 117,500 | 85.7 ± 14.3 (34/39, N = 7) | 83.3 ± 14.1 (33/39, N = 7) | 97.1 |
| 880 | 117,500 | 91.7 ± 8.3 (31/34, N = 6) | 86.1 ± 8.0 (29/34, N = 6) | 93.5 | |
| 95 | 117,500 | 100 ± 0.0 (25/25, N = 5) | 92.0 ± 5.5 (23/25, N = 5) | 92 | |
| 0.5× | 69,000 | 4,050 | 34.3 ± 14.3 (12/35, N = 7) | 14.3 ± 14.3 (5/35, N = 7) | 41.7 |
| 880 | 4,050 | 30.0 ± 12.0 (10/32, N = 6) | 12.8 ± 9.8 (4/32, N = 6) | 40 | |
| 95 | 4,050 | 31.1 ± 11.8 (10/32, N = 6) | 21.1 ± 8.4 (7/32, N = 6) | 70 | |
| 0.33× | 69,000 | 117,500 | 74.8 ± 11.5 (29/39, N = 7) | 39.5 ± 14.2 (15/39, N = 7) | 51.7 |
| 880 | 117,500 | 48.0 ± 21.2 (12/28, N = 6) | 40.0 ± 24.5 (10/28, N = 6) | 83.3 | |
| 95 | 117,500 | 28.6 ± 17.8 (10/35, N = 7) | 10.0 ± 10.0 (3/30, N = 6) | 30 | |
| 0.33× | 69,000 | 4,050 | 0.0 ± 0.0 (0/20, N = 4) | 0.0 ± 0.0 (0/20, N = 4) | 0 |
| 880 | 4,050 | 5.0 ± 10.0 (1/20, N = 4) | 0.0 ± 0.0 (0/20, N = 4) | 0 | |
| 95 | 4,050 | 40.0 ± 23.1 (10/25, N = 5) | 8.0 ± 4.9 (2/25, N = 5) | 20 | |
The data are % survival ± SEM. The first sets of parentheses are the ratios of the number of surviving oocytes to the number frozen or vitrified. N is the number of replicates.