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. Author manuscript; available in PMC: 2015 Feb 20.
Published in final edited form as: Chem Biol. 2014 Jan 2;21(2):238–245. doi: 10.1016/j.chembiol.2013.12.004

Figure 5.

Figure 5

Exponential growth deduced from the initial rate of reaction as a function of the starting concentration of enzyme. Reactions were carried out using either 5 (gray) or 10 (black) μM substrates. Initial velocity was measured over the first 10% of the reaction, and the data were fit to an equation with reaction order 1.0. Reaction conditions: 25 mM MgCl2, pH 8.5, 48 °C.