Table 1.

Parameters for Successive Rounds of In Vitro Evolution

Round	Formata	Enzyme (pmol)b	[Substrate] (nM)	Time (min)
1	A	830	250	60
2	A	200	100	5
3	A	100	50	2.5
4	B	10	2	0.5
5	B	10	2	0.5
6	A	25	50	1
7	A	25	50	1
8	A	25	25	0.5
9	A	5	5	0.08
10	A	5	5	0.08

^aReaction with either A or B as the separate substrate.

^bConcentration of enzyme was half that of substrate in rounds 1–8 and equal to that of substrate in rounds 9 and 10.