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. 2014 Mar 5;9(3):e90277. doi: 10.1371/journal.pone.0090277

Figure 2. Hypothalamic-like differentiation triggered ELS-responsive DNA methylation at the Avp enhancer.

Figure 2

A, ELS-responsive DNA methylation rapidly evolved following 4 days of Lif withdrawal and was maintained during subsequent cyclopamine treatment as evidenced by bisulfite sequencing of 25 clones. Shaded hemispheres represent percentage of methylation at each ELS-responsive CpG residue. Results are representative of three independent experiments. B, Comparison of ELS-responsive DNA methylation at the Avp enhancer in the SON, PVN and following hypothalamic-like differentiation of EB5 cells. C, Overall CpG methylation at CpG island 3 (CGI3) comprising the ELS-responsive Avp enhancer in the developing mouse hypothalamus. Postnatal days (PND) are indicated. D, Avp mRNA expression as evidenced by qRT-PCR analysis in the developing mouse dorsal hypothalamus. Bars represent standard deviations from the mean (sem), from 4–5 independent experiments.