Figure 1. Percent survival and ELISA detection of antibody levels in immunized mice.

(A) Female BALB/c mice were immunized with rSEB plus AlPO₄ adjuvant, AlPO₄ adjuvant alone or PBS alone before MRSA252 infection. Two weeks after the last immunization, mice were challenged with 8.4×10⁸ CFU of MRSA252 by intravenous injection. The percent survival was analyzed in mice after the MRSA challenge. (B) To determine the anti-SEB titers in immunized animal serum samples, microtiter plates were coated with native SEB. Sera from immunized mice were analyzed using an ELISA. The antibody levels in immunized mice with rSEB plus AlPO₄ adjuvant, AlPO₄ adjuvant alone or PBS alone are shown.