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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Biol Inorg Chem. 2013 Nov 30;19(3):319–334. doi: 10.1007/s00775-013-1068-3

Figure 2.

Figure 2

Representative plots of titration data showing the thermal effect of 55 × 5 μL injections of Ni2+ onto a solution of HpUreF. In A, raw heat data are shown for wild type HpUreF. In B, integrated heat data of wild type HpUreF (black filled circles), H229A HpUreF (blue filled circles) mutant, C231A HpUreF mutant (red filled circles) and H229A/C231A HpUreF double mutant (hollow circles) are indicated together with the best fits (solid lines).