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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Biol Inorg Chem. 2013 Nov 30;19(3):319–334. doi: 10.1007/s00775-013-1068-3

Figure 6.

Figure 6

(A) Scheme of the pGEM::ureOP plasmid containing the 6.1 kbp H. pylori urease operon utilized for in cell studies; (B) Urease activity measured for the soluble cellular extract of E. coli BL21(DE3) cells transformed with the pGEM::ureOP plasmid and with its H229A-HpUreF, C231A-HpUreF and H229A/C231A-HpUreF mutants. The enzymatic activities, measured in duplicate on two different cultures, are normalized to the total protein content of bacterial lysate.