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. 2014 Mar 5;9(3):e90413. doi: 10.1371/journal.pone.0090413

Figure 1. Diagrammatic representation of the DNA constructs, including control plasmids, used for immunization.

Figure 1

The construct for P. yoelii used mouse MIP3α, while the P. falciparum construct employed the sequence for human MIP3α. M refers to MIP3α. CSP refers to a segment of the Plasmodium yoelii circumsporozoite protein; and MCSP refers to the fusion protein of MIP-3α and a segment of the P. yoelii circumsporozoite protein. Also shown are the positions of the leader sequence, spacer, and the myc tag. The leader sequence for the P. yoelii construct is the interferon–γ induced protein 10 secretion leader sequence; the leader sequence for the P. falciparum construct is the human tissue plasminogen activator leader sequence [46]. The MCSP and hMfCSP nucleotide sequences are provided in the supplementary materials.