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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Pediatr. 2013 Dec 5;164(3):566–571. doi: 10.1016/j.jpeds.2013.10.063

Figure 2. Assessment of ECFC phenotype.

Figure 2

A, ECFCs express CD31, vWF, VE-Cadherin, and uptake Dil-Ac-LDL. Cell nuclei were counterstained with DAPI (scale bar: 50 μm). B, Quantitative RT-PCR analyses of ECFCs for endothelial (CD31, vWF, VE-Cadherin, VEGFR2, eNOS) and mesenchymal (CD90, PDGFRβ) markers. C, Flow cytometric analysis of ECFCs for CD31, CD90, and CD45 (black line histograms). Isotype-matched controls are overlaid in solid gray histograms. D, Cloning-forming ability of ECFCs. The endothelial nature of the colonies was confirmed by binding of UEA-1 lectin (scale bar: 500 μm). E, Outgrown colonies were categorized by size. Bars represent mean±SE (n=6).