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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Pediatr. 2013 Dec 5;164(3):566–571. doi: 10.1016/j.jpeds.2013.10.063

Figure 3. Assessment of ECFC function.

Figure 3

ECFCs were categorized into pre-pregnancy maternal BMI <25 kg/m2 and 25–30 kg/m2. A, Cell proliferation in response to VEFG-A (10 ng/mL) and FGF-2 (1 ng/mL). B, Cell migration in response to VEGF-A and FGF-2 expressed as percentage of gap closure. C, Capillary-like network formation on Matrigel expressed as total tube length per field. D, Adhesion of leukocytes onto ECFCs after stimulation with TNF-α. E, Vasculogenic properties of ECFCs compared in nude mice after subcutaneous implantation. H&E staining reveals numerous blood vessels (yellow arrowheads; scale bar: 100 μm). F, Human-specific lumens confirmed by staining with UEA-1 (red) and perivascular coverage indicated by α-SMA (green) (white arrowheads; scale bars: 50 μm). G, Microvessel density. H, ECFCs expressing UEA-1 and human CD31. Bars represent mean±SE (n=3 each group).