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. 2014 Mar 5;9(3):e90429. doi: 10.1371/journal.pone.0090429

Figure 2. Flow chart of the blood contamination studies.

Figure 2

Cerebrospinal fluid (CSF) from four patients (P1-P4) were pooled and divided into aliquots which were spiked with different amounts of blood. In experiment A, a reference sample was compared to CSF spiked to achieve 0.5% and 2% blood. The CSF samples were centrifuged, protein depleted, and separated by SDS-PAGE into ten fractions per lane. In experiment B, we examined the effect of centrifugation. A reference sample was compared to two CSF samples spiked to achieve 0.5% blood, where one of the blood contaminated samples was not centrifuged. The samples were trypsin digested in-solution. All samples were analyzed on an OrbiTrap Velos Pro.