Figure 5.

Colon histology, pathology scores and number of apoptotic and mitotic figures per crypt in 50-day-old mice. Panels A-C are representative H&E-stained colon sections from a male DKO (A), TKO (B) and WT Nox- (C) mice. The white arrow points out a slightly distorted gland with mucin depletion. DKO mice had few goblet cells, which are present in TKO (DKO Nox1−) and abundant in the WT Nox1− mouse. Panel D. A box-and-whisker plot of colon pathology score showing the median and interquantile range. The scores were determined from 20 male DKO, 20 male TKO, 13 female DKO, 11 female het-TKO (DKO Nox1+/−), 4 WT and 7 Nox1-KO mice. Different letters above the boxes indicate different medians (Panel D), where a>b>c. Groups that share the same letter are not different; i.e. ab is not different from a, and bc is not different from c. Panel E. A column graph showing the mean of apoptotic figures per crypt in mouse colon. The number of mice in each group is 16 male DKO, 15 male TKO, 9 female DKO, 5 female het-TKO, 3 WT and 6 Nox1-KO. Groups with different letters are significantly different, where a>b. Panel F. A column graph of mean mitotic figures per crypt in mouse colon analyzed from the same number of mice in Panel E, except male DKO group has 17 mice. The error bars in E and F are one SD.