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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Immunol. 2014 Jan 29;192(5):2349–2356. doi: 10.4049/jimmunol.1302982

Figure 1. Role of 5-LO metabolites in the expression of pro-inflammatory microRNAs in macrophages.

Figure 1

(A) Thioglycollate-elicited macrophages from WT and 5-LO−/− mice were harvested, and total RNA was isolated. MicroRNAs were isolated and subjected to miScript cDNA synthesis and to real time PCR array as described in Materials and Methods. Data are representative of 5 individual experiments. (B) Detection of individual microRNAs in WT and 5-LO−/− macrophages. Detection of miR-155, Let-7g and miR-146b was performed as described in Materials and Methods. (C) WT MΦs treated with the 5-LO inhibitor AA-861, the BLT1 antagonist U7532 and the cysLT1 antagonist MK571 (all at 1 μM) for 24 h, followed by determination of the indicated microRNAs. Data are mean ± SEM from 3 individual experiments, each performed in triplicate. *P < 0.05 versus untreated WT; #P < 0.05 versus WT control or LTB4 alone.