Table 2.

Genes duplicated relative to A. burtoni with no informative BLAST hit

GenBank	A.twe	M.est	P.sim	R.chi
CN4691252/2	1.32	ns	ns	ns
	2E-5
CN469431	ns	ns	ns	0.50
				0.0017
CN469460	ns	ns	0.64	ns
			0.0007
CN469913	ns	ns	ns	0.63
				0.0008
CN470216	ns	0.39	ns	ns
		0.0039
CN470402	ns	ns	0.48	0.45
			0.0034	0.0051
CN470540	ns	ns	ns	0.60
				0.0051
CN470597	0.65	ns	ns	ns
	0.0013
CN470646	0.73	ns	ns	ns
	0.0003
CN470701	ns	ns	ns	0.65
				0.0052
CN470713	ns	ns	0.68	ns
			0.0009
CN4707241/2	1.02	ns	ns	ns
	0.0027
CN470781	0.58	ns	ns	ns
	0.0025
CN470835	ns	1.55	ns	ns
		0.0002
CN470857	ns	0.67	ns	0.96
		0.005		0.0006
CN470988	ns	1.28	ns	1.34
		0.0007		0.0005
CN471261	ns	ns	0.93	ns
			0.001
CN471811	ns	1.35	1.22	ns
		6E-6	1E-5
CN471851	ns	ns	0.47	ns
			0.0018
CN472050	ns	ns	ns	0.70
				0.0002
DY625804	ns	1.23	ns	ns
		0.0001
DY6258451/2	1.16	ns	ns	ns
	6E-6
DY625884	0.49	ns	ns	ns
	0.0024
DY625919	1.18	ns	ns	ns
	0.0001
DY626122	1.50	ns	ns	ns
	2E-5
DY626140	ns	1.05	ns	ns
		0.0036
DY626192	ns	0.75	ns	ns
		0.0002
DY626304	ns	ns	0.99	1.50
			4E-5	2.2E-6
DY626389	ns	ns	0.57	ns
			0.0022
DY626428	ns	ns	ns	0.39
				0.0053
DY626737	ns	ns	0.79	ns
			0.0001
DY626766	ns	0.81	0.98	0.68
		0.0003	0.0001	0.0009
DY627085	ns	0.44	ns	ns
		0.0043
DY627087	1.15	ns	ns	ns
	3E-5
DY627338	0.72	ns	ns	ns
	0.0006
DY627361	ns	ns	ns	0.74
				0.0001
DY627641	ns	0.76	0.85	ns
		0.0012	0.0006
DY627780	ns	1.51	ns	ns
		3E-6
DY627911	1.04	0.87	0.49	ns
	5E-5	0.0002	0.0046
DY628148	0.50	ns	ns	ns
	0.0012
DY628172	1.38	ns	ns	ns
	4E-6
DY628268	ns	ns	0.46	ns
			0.0017
DY628316	ns	0.64	ns	ns
		0.0026
DY628477	ns	ns	ns	0.58
				0.0052
DY628517	0.76	ns	ns	ns
	0.0002
DY628561	ns	ns	ns	0.42
				0.0052
DY628642	ns	ns	1.62	1.13
			9E-5	0.0087
DY6287021/2	ns	ns	ns	0.67
				0.0004
DY628714	ns	ns	ns	0.48
				0.0027
DY629058	ns	ns	ns	2.41
				3.7E-5
DY629123	ns	0.87	0.83	1.41
		0.0001	0.0001	3.8E-6
DY629387	ns	ns	1.18	ns
			4E-5
DY629482	1.39	0.71	ns	1.12
	1E-5	0.0008		4.3E-5
DY629717	ns	ns	1.60	1.28
			0.0002	0.0007
DY6298821/2	ns	ns	ns	0.77
				0.0032
DY629912	1.41	2.21	1.06	1.16
	8E-6	0.0003	7E-6	1.1E-6
DY630229	ns	0.88	0.83	ns
		0.0015	0.0021
DY630284	ns	0.54	ns	ns
		0.0032
DY6303731/2	ns	0.89	1.00	1.23
		0.0001	6E-5	1.5E-5
DY630867	0.97	0.64	ns	ns
	0.0002	0.0024
DY630964	ns	ns	ns	0.95
				0.0037
DY630993	ns	0.67	ns	ns
		0.002
DY631067	ns	0.78	0.80	1.02
		0.0008	0.0007	0.0002
DY631315	ns	ns	1.57	1.16
			0.003	0.0019
DY631408	ns	ns	0.50	ns
			0.0033
DY631442	ns	1.40	1.16	ns
		0.0014	0.004
DY6315051/2	ns	0.72	ns	ns
		0.0029
DY631507	0.67	0.69	0.78	0.61
	0.0013	0.0011	0.0005	0.0022
DY631680	ns	ns	ns	1.02
				0.0019
DY631698	ns	ns	1.03	ns
			0.0006
DY631821	ns	ns	0.61	0.60
			0.0038	0.0039
DY631827	ns	0.86	ns	ns
		0.0046
DY631850	0.85	ns	ns	ns
	0.0009
DY631869	ns	0.39	ns	ns
		0.0038
DY632007	0.99	ns	ns	ns
	0.0002
DY6320583/3	ns	0.90	0.72	0.71
		0.0007	0.0026	0.0028
DY632092	ns	1.09	ns	ns
		0.0007
DY632097	ns	0.79	ns	0.82
		0.0031		0.0002
DY6321342/2	0.94	0.86	ns	0.84
	0.0002	0.0003		0.0003
DY632256	ns	ns	1.56	ns
			0.0033
DY632294	ns	0.41	ns	ns
		0.0035

Genomic Content Value: Log2 Hybridization coefficients for each species relative to A. burtoni as estimated by the linear model following background correction and normalization followed by the uncorrected p-value for those significantly different. A.twe: A. tweddlei; M.est: M. estherae; P.sim: P. similis; R.chi: R. chilingali; “ns”: not significant; “*”: the GenBank number is a representative for multiple array features for that gene.