Table 3.
Oligonucleotide primers used for qPCR designed against GenBank sequence available for microarray features
| GenBank | Primer sequence | Homology | |
Primer efficiency |
|
|---|---|---|---|---|---|
| Predicted length | A. burtoni | Test species | |||
|
DY626766 |
F: TCGGTCTCCTTAACCGGATG |
No Hit |
193 |
86 |
74 |
| |
R: CTGAGTTTGGCTGCCCGTAA |
|
|
|
(P. similis) |
|
DY627986 |
F: ACGAACACCCGAACGGAAAC |
Hox gene cluster |
222 |
100 |
104 |
| |
R: GGTGCACGCACATGAACTGT |
|
|
|
(M. estherae) |
|
DY631898 |
F: CGTCCCAGTGAGGATGAGGA |
MHC class II antigen |
161 |
82 |
82 |
| |
R: TGATGCTGATCGGTTGATGC |
|
|
|
(R. “chilingali”) |
|
DY632057 |
F: ATTACTGCGAGTGCCGTCCA |
Pituitary adenylate cyclase activating |
150 |
91 |
78 |
| R: CTGCGCCCTGAAAGAACAGA | polypeptide receptor 1A | (A. tweddlei) | |||
Primer Efficiency: percent is based on 4-fold template dilutions for A. burtoni and one heterologous test species indicated in parentheses.