Figure 6. Quantification of cellular viability in a dose and time-dependent manner.

Infection experiments with Vero and PLC/PRF/5 (at MOIs of 1 and 0.1) cells were performed with all variants of the newly generated Sendai viruses as well as the wild type variant SeV D52. Cellular viability was investigated via CytoTox-Glo™ assay at different time points post infection (0 h, 24 hpi, 48 hpi, 72 hpi, 96 hpi). The assay was performed in triplicates and repeated three times; data are shown as mean and SEM. SeV 1 (green line): SeV D52, SeV 2: SeV Fmut, SeV 3: SeV FmutV_ko, SeV 4: SeV FmutC_ko, 5: SeV FmutY_ko, 6: SeV FmutC_koV_ko, 7: SeV FmutC/Y_termV_ko, TX: Triton X-100 (positive control for the induction of a maximum grade, chemically-mediated destruction of test cells).