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. 2014 Mar 5;9(3):e90508. doi: 10.1371/journal.pone.0090508

Figure 7. Cytolytic capacity of recombinant SeV particles in different cell types.

Figure 7

Cell growth and cell lysis in different cell types with SeV D52 and six recombinant SeV Fmut variants (black bars: MOI 0.01, grey bars: MOI 0.1). (A+C) Analysis of cell mass by sulforhodamine B (SRB) assay 72 hpi in (A) hepatoma and (C) non-malignant MRC-5 cells or PHH. All values are shown in relation to uninfected control cells (mock). (B+D) Analysis of LDH release of infected cells relative to LDH release of uninfected control cells (mock) as a surrogate marker of cellular membrane integrity 72 hpi. Results represent mean and SD of three independent experiments in triplicates, for PHH two different donors, in triplicates each.