Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 5;9(3):e90508. doi: 10.1371/journal.pone.0090508

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Zimmermann et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Recombinant SeV variants (SeV D52, SeV Fmut, SeV FmutC_koV_ko, SeV FmutC/Y_termV_ko, 1×10⁷ TCID₅₀/100 µl) were injected intratumorally in tumors of a PLC/PRF/5 hepatoma xenograft mouse model. (A) 48 h post injection the mice were sacrificed, tumors were removed and one quarter of each tumor was fixed and embedded in paraffin. Virus spread was investigated applying an anti-GFP antibody for immunohistochemistry analysis (brown color). Bar represents 100 µm. (B) Indicator cultures (Vero cells) were infected with lysates from frozen tissue sections (one quarter of tumor, liver, spleen, heart, lung, each). Early primary infections (24-72 hpi) were observed and single infected cells were counted as initial virus particles (VP). Fluorescence microscopy pictures of the infected Vero cells were taken 72 hpi. Shown are representative picture for each animal. Bar represents 400 µm.