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. Author manuscript; available in PMC: 2015 Feb 21.
Published in final edited form as: ACS Chem Biol. 2013 Nov 25;9(2):334–338. doi: 10.1021/cb400704n

Figure 1. Quantitative, time-resolved proteomic analysis of miR-126 over-expression by combining BONCAT and SILAC.

Figure 1

Cells were cultured in SILAC medium containing either L-lysine (light) or [13C6, 15N2]L-lysine (heavy, lys8) for five doubling times prior to induction of miR-126 expression for 24 h, followed by a 4 h Aha pulse. Lysates from the two cultures were mixed at equal protein concentrations and conjugated to an alkyne-biotin tag. Newly synthesized proteins were enriched prior to mass spectrometry analysis.