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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Oral Oncol. 2013 Nov 8;50(2):120–127. doi: 10.1016/j.oraloncology.2013.10.013

Figure 1. Obatoclax inhibits growth activity of HNSCC cells.

Figure 1

Figure 1

Figure 1

A: Chemical structure of obatoclax

B: UMSCC-1, Cal33, 1483, and UMSCC-22A were grown in 6-well plates for 48 hours. Whole cell lysates were prepared and subjected to immunoblotting for MCL-1, BCL-2 and BCL-XL; β-actin was used as a loading control. Ratios were calculated by densitometric analysis.

C: HNSCC cell lines were treated with 0.1% DMSO or increasing concentrations of obatoclax (1 nM -10 000 nM) for 48 hours. Growth inhibition was assessed by MTT assays. Each experiment was performed at least three independent times, and the values represent the mean (± SEM).