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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Oral Oncol. 2013 Nov 8;50(2):120–127. doi: 10.1016/j.oraloncology.2013.10.013

Figure 3. Obatoclax induces apoptosis in HNSCC cells.

Figure 3

Figure 3

Figure 3

A: UMSCC-1 and UMSCC-22A were treated for 48 hours with 0.1% DMSO or obatoclax (100 or 200 nM. Cell cycle distributions were determined by flow cytometric analyses, as described in Materials and Methods. The data shown is representative of 3 independent experiments.

B: UMSCC-1 cells were treated for 48 hours with 0.1% DMSO or obatoclax, followed by flow cytometric determination of Annexin V staining. Columns represent the average percentage of Annexin-V positive cells from three independent experiments; error bars represent SEM.

C: UMSCC-1 and UMSCC-22A were treated with 0.1% DMSO or obatoclax for 48 hours, followed by immunoblotting with anti-PARP. β-actin was used as a loading control. Full-length PARP (PARP) and cleaved PARP (c-PARP) are depicted. Data shown are similar to those obtained in three independent experiments.