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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Metab Brain Dis. 2013 Dec 28;29(1):59–73. doi: 10.1007/s11011-013-9474-3

Figure 1. Adoptive transfer of IL10+ B-cells, 24 hours before or 4 hours after MCAO, reduces infarct volume in male WT mice.

Figure 1

A) Intravenous transfer of 5 million IL10+ B-cells given 24 hours before surgery to induce middle cerebral artery occlusion (MCAO) reduced infarct volume in C57BL/6J (wild-type, WT) mice (n=10), 96 hours following 60 minutes of MCAO compared to intravenous transfer of RPMI vehicle (no cells) (n=12). *P<0.05 B) Representative 2,3,5 triphenyltetetrazolium chloride stained cerebral sections 96 hours following 60 minutes of MCAO. Localization of the ischemic lesion differed between WT mice receiving intravenous IL10+ B-cells (right column) vs. RPMI vehicle (no cells)(left column) 24 hours before MCAO. C) Intravenous transfer of 5 million IL10+ B-cells 4 hours after surgery to induce MCAO reduced cortical and hemispheric (total) infarct volume in WT mice (n=13), 96 hours following 60 minutes of MCAO compared to intravenous transfer of RPMI vehicle (no cells) (n=14),. *p<0.05; **p<0.01 D) Representative 2,3,5 triphenyltetetrazolium chloride stained cerebral sections 96 hours following 60 minutes of MCAO. Localization of the ischemic lesion differed between WT mice receiving intravenous IL10+ B-cells (right column) vs. RPMI vehicle (no cells)(left column) 4 hours after MCAO.