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. Author manuscript; available in PMC: 2015 Feb 21.
Published in final edited form as: ACS Chem Biol. 2013 Dec 9;9(2):517–525. doi: 10.1021/cb4007776

Figure 2. Assay development and application in 96-well format.

Figure 2

(a–c) Development using MBP. (d–f) Application to myoc-OLF. (a, d) Chemical melt with addition of GdnHCl monitoring SO fluorescence. (b, e) Serial dilution to optimize protein concentration for subsequent assays. (c, f) Dose-dependent stabilization by ligands for MBP (red, maltose; green, maltotetraose; blue, maltitol) (c) or TMAO for myoc-OLF (f) as monitored by SO fluorescence. Error bars denote standard deviation, arrows at selected concentrations.