Table 1.
Summary of compounds identified in myoc-OLF pilot screen by CARS and binding by SPR
| Compound | ΔTm(°C) | Kd (µM) from SPR |
|---|---|---|
| Apigenin | −0.3 ± 0.2 | 41.6 ± 39.6c |
| Aurintricarboxilic acid | 0.3 ± 0.2 | 17.1 ± 9.6 |
| GW5074 | 0.2 ± 0.0 | 33.5 ± 20.9d |
| Tyrphostin AG 879 | −0.8 ± 0.0 | 38.6 ± 12.7c |
| Myricetin | 1.7 ± 0.0 | linear dose-response |
| Isoliquiritigenin | 1.3 ± 0.1 | linear dose-response |
| Rottlerin | N/Af | 30.1 ± 0.6e |
| Phloretin | 1.2 ± 0.0 | linear dose-response |
| Piceatannol | −0.4 ± 0.1 | sigmoidal dose-response |
| Morin | −0.4 ± 0.4 | weak sigmoidal responsec,d |
| Niclosamide | 0.0 ± 0.2 | no detectable bindingd |
| Quercetin dihydratea | N/A | N/A |
| Reactive Blue 2a | N/A | N/A |
| (R,R)-cis-diethyl tetrahydro-2,8-chrysenediola | N/A | N/A |
a
Not tested.
c
Result obtained with surfactant P-20 in buffer solution.
d
Poor solubility.
e
Non-stoichiometric, promiscuous binder.
N/A = not available for further analysis.