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. Author manuscript; available in PMC: 2015 Feb 21.
Published in final edited form as: ACS Chem Biol. 2013 Nov 25;9(2):433–442. doi: 10.1021/cb4006886

Figure 1.

Figure 1

VopS reaction and assay design. a) AMPylators transfer AMP to side chain threonines or tyrosines. b) FP assay design. A time dependent increase in FP signal occurs as VopS transfers Fl-AMP to Cdc42. In the presence of an inhibitor, however, no Fl-AMP is transferred and the FP signal is diminished. c) Structure of Fl-ATP. d) Substrates (Cdc42 and Fl-ATP) were added to 16 replicates of VopS, VopS with 0.4 mM ATP or VopS Screening Buffer in a 384-well plate. FP was measured as a function of time.