Table 2. Antigenic analysis of H5N1 influenza viruses from Bangladesh in the hemagglutination inhibition assays.
| H5N1 antigen |
α-H5N1 (post-infection ferret antisera) | |||||||
|---|---|---|---|---|---|---|---|---|
| Reference antigens | Clade | α-BHG/QH/1A | α-Hubei/1 | α-BS/HK/1161* | α-CM/HK/5052 | α-Ck/BD/15205§ | α-Env/BD/15121*,§ | α-Dk/BD/19097*,§ |
| RG-A/BAR-HEADED GOOSE/QUNGHAI/1A/2005 | 2.2 | 640 | 80 | 320 | 80 | 80 | 640 | 160 |
| RG-A/HUBEI/1/2010 | 2.3.2.1 | 40 | 640 | 2560 | 320 | 640 | 5120 | 1280 |
| RG-A/BARN SWALLOW/HONG KONG/1161/2010 | 2.3.2.1 | 40 | 160 | 5120 | 640 | 640 | 5120 | 1280 |
| RG-A/COMMON MAGPIE/HONG KONG/5052/2007 | 2.3.2.1 | 40 | 160 | 1280 | 640 | 320 | 2560 | 640 |
| A/CHICKEN/BANGLADESH/15205/2012 | 2.3.2.1 | 40 | 80 | 1280 | 320 | 640 | 2560 | 2560 |
| A/ENVIRONMENT/BANGLADESH/15121/2012 | 2.3.2.1 | 40 | 80 | 1280 | 320 | 320 | 2560 | 1280 |
| A/DUCK/BANGLADESH/19097/2013 | 2.3.2.1 | 40 | 160 | 2560 | 640 | 1280 | 5120 | 2560 |
| TEST ANTIGENS | ||||||||
| A/CHICKEN/BANGLADESH/9636/2010 | 2.2.2 | 640 | 160 | 320 | 80 | 640 | 640 | 320 |
| A/CHICKEN/BANGLADESH/9675/2011 | 2.2.2 | 320 | 80 | 80 | 40 | 320 | 320 | 160 |
| A/CHICKEN/BANGLADESH/12197/2011 | 2.3.2.1 | 40 | 80 | 1280 | 320 | 320 | 2560 | 640 |
| A/CHICKEN/BANGLADESH/15078/2012 | 2.3.2.1 | 40 | 80 | 1280 | 160 | 320 | 1280 | 640 |
| A/CHICKEN/BANGLADESH/15079/2012 | 2.3.2.1 | 20 | 160 | 640 | 320 | 320 | 2560 | 640 |
| A/CHICKEN/BANGLADESH/15083/2012 | 2.3.2.1 | 40 | 160 | 1280 | 320 | 320 | 2560 | 640 |
| A/CHICKEN/BANGLADESH/15085/2012 | 2.3.2.1 | 10 | 160 | 640 | 320 | 320 | 1280 | 640 |
| A/ENVIRONMENT/BANGLADESH/15105/2012 | 2.3.2.1 | 10 | 40 | 1280 | 320 | 320 | 2560 | 640 |
| A/ENVIRONMENT/BANGLADESH/15114/2012 | 2.3.2.1 | <10 | 80 | 1280 | 160 | 320 | 2560 | 640 |
| A/ENVIRONMENT/BANGLADESH/15126/2012 | 2.3.2.1 | 20 | 80 | 1280 | 160 | 320 | 5120 | 640 |
| A/ENVIRONMENT/BANGLADESH/15145/2012 | 2.3.2.1 | 10 | 160 | 2560 | 320 | 640 | 5120 | 1280 |
| A/ENVIRONMENT/BANGLADESH/15147/2012 | 2.3.2.1 | 20 | 80 | 1280 | 320 | 320 | 5120 | 1280 |
| A/ENVIRONMENT/BANGLADESH/15165/2012 | 2.3.2.1 | 10 | 160 | 5120 | 640 | 640 | 5120 | 1280 |
| A/CHICKEN/BANGLADESH/15210/2012 | 2.3.2.1 | 20 | 160 | 640 | 80 | 640 | 2560 | 640 |
| A/ENVIRONMENT/BANGLADESH/15661/2012 | 2.3.2.1 | 10 | 80 | 1280 | 320 | 640 | 2560 | 640 |
| A/ENVIRONMENT/BANGLADESH/16298/2012 | 2.3.2.1 | 10 | 160 | 1280 | 320 | 640 | 2560 | 1280 |
| A/DUCK/BANGLADESH/17753/2012 | 2.3.2.1 | 40 | 160 | 1280 | 320 | 320 | 2560 | 640 |
| A/CHICKEN/BANGLADESH/18061/2012 | 2.3.2.1 | 40 | 40 | 640 | 80 | 320 | 2560 | 640 |
| A/CHICKEN/BANGLADESH/18065/2012 | 2.3.2.1 | 40 | 80 | 640 | 80 | 320 | 2560 | 640 |
| A/CHICKEN/BANGLADESH/18248/2012 | 2.3.2.1 | 10 | 160 | 1280 | 320 | 640 | 2560 | 640 |
| A/ENVIRONMENT/BANGLADESH/18948/2013 | 2.3.2.1 | 20 | 80 | 1280 | 320 | 640 | 2560 | 640 |
| A/DUCK/BANGLADESH/18949/2013 | 2.3.2.1 | <10 | 160 | 2560 | 320 | 640 | 5120 | 1280 |
| A/CHICKEN/BANGLADESH/18984/2013 | 2.3.2.1 | 20 | 40 | 640 | 40 | 320 | 2560 | 640 |
| A/ENVIRONMENT/BANGLADESH/18989/2013 | 2.3.2.1 | 20 | 40 | 320 | 40 | 160 | 1280 | 320 |
| A/DUCK/BANGLADESH/19099/2013 | 2.3.2.1 | <10 | 40 | 640 | 160 | 320 | 1280 | 320 |
| A/QUAIL/BANGLADESH/19250/2013 | 2.3.2.1 | <10 | 20 | 160 | 40 | 40 | 320 | 160 |
| A/QUAIL/BANGLADESH/19254/2013 | 2.3.2.1 | 40 | 10 | 160 | 40 | 40 | 320 | 80 |
| A/ENVIRONMENT/BANGLADESH/19338/2013 | 2.3.2.1 | <10 | 20 | 320 | 40 | 80 | 640 | 160 |
§indicates sera that have been produced against recent highly pathogenic H5N1 viruses from Bangladesh.
*During serum production, ferrets were boosted with antigen in incomplete Freund's adjuvant on day 14 post-inoculation.