Fig. 3.

Up-regulation of IFN-γ-dependent CXCR3 chemokine, IP-10, in response to radiation treatment is dependent on endogenous type I IFNs and IFN-γ. WT and IFNABR KO (a, c) or IFNγKO (b, d) mice were inoculated with B16 melanoma cells and treated with 15 Gy local radiation as before. a, b Total RNA was isolated from tumors on day 6 post-RT. Absolute qRT-PCR was performed as described in Materials and Methods. c, d Tumors were excised on day 7 post-RT, homogenized, and IP-10 ELISA was used to determine IP-10 protein levels within each tumor. Each value was normalized to total protein concentration in each individual sample. Data shown are representative of two independent experiments (n = 4, *p < 0.05, **p < 0.01, ***p < 0.001)