Table 1. Examples of Nanog antibodies and their recognized protein bands.
| Antibody* | Remarks | Protein band(s) | Reference(s) |
| eBioscience mAb (Cat# 14-5768) | Affinity-purified mAb using full-length hNanog as immunogen | ∼43 kD band in N-tera lysate; 2 bands of unspecified M.W | Product sheet, 24 |
| Kamiya Rb pAb (PC-102) | Affinity-purified pAb using hNanog aa 29-49 as immunogen | ∼42 kD band in hESCs | Product sheet |
| SC goat pAb-N17 (SC-30331; N17) | Affinity-purified pAb against a hNanog N-terminus peptide | ∼43 kD band using rhNanog;∼34 kD in cancer cell lysates | Product sheet, 25 |
| Cell Signaling pAb (3580) | Affinity-purified Rb pAb against N-terminus of hNanog | 42∼45 kD band in N-tera lysate | Product sheet |
| Cell Signaling mAb (5232) | Affinity-purified Rb mAb against N-terminus of hNanog | This antibody is used for ChIP | Product sheet |
| BioLegend Rb pAb (632002) | Affinity-purified pAb against hNanog aa 135-149 | ∼46 kD band in N-tera cells | Product sheet |
| SC Rb pAb-H155 (SC-33759; H155) | Using hNanog aa151-305 peptide as immunogen | 40 kD band in human ES cells; ∼40 kD in adipose-derived stem cells; ∼40 kD in lymphoma cell lines. | Product sheet, 47, 33 |
| R & D goat pAb (AF1997) | Affinity-purified pAb against rhNanog aa 153-305 peptide | 40∼42 kD band using rhNanog; 35 kD band in hESCs & EC cells; Several bands at ≥34 kD in HepG2 & OS732 cells; 36∼37 kD in trophoblastic samples; ∼42 kD in human colorectal cancer | Product sheet, 5, 16, 48, 26, 46 |
| Abcam Rb pAb (21624) | Same as Kamiya pAb | ∼40 kD band in hESCs; A cluster of bands (unknown MW) | Product sheet, 49 |
| Abcam Rb pAb (21603) | Using full-length mNanog as immunogen | ∼39 kD band in mESCs A cluster of bands (unknown MW) ∼38 kD band in hESCs | Product sheet, 50, 51 |
| Abnova mouse mAb (Clone 2C11) | Raised against C-terminal Nanog | 48 kD, 35 kD and 29 kD bands in N-tera cells | 52 |
| Bethyl Labs Rb pAb (BL1662) | Affinity purified pAb raised against mNanog | At least two bands between 37 and 49 kD in mESCs | 53 |
| Chemicon Rb pAb (AB5731) | Affinity purified pAb raised against mNanog N-terminus peptide | ∼35 and 55 kD bands in mESCs; A cluster of bands in mESCs | Product sheet, 54 |
| Chemicon Rb pAb | Raised against mNanog | 40 kD and 80 kD bands in mESCs; Dog testis and pig testis | 55 |
| Home-made Rb pAb | Raised against mNanog | 2-3 bands at ∼40 kD in mESCs | 2 |
| Home-made Rb pAb | Raised against mNanog | >3 bands with the largest at ∼44 kD in mESCs, and EG cells | 56 |
| Home-made Rb pAb | Raised against hNanog (aa 168-183) | >3 bands of ∼36 kD to ∼50 kD in 293T cells transfected with hNanog | 57 |
| Home-made Rb pAb | FLAG-tagged Nanog stably expressed in mESCs | Two pulldown Nanog protein bands at ∼45 kD and 37 kD | 58 |
| Home-made Rb pAb | Raised against mNanog aa 1-95 | Several bands at >35 kD | 59 |
*This table lists the information for the 8 antibodies used in our current study, together with several others commercial and home-made antibodies. Shown in parenthesis are catalog numbers.
Abbreviations: EC, embryonal carcinoma; EG, embryonic germ cells; hESCs, human embryonic stem cells; hNanog,
human Nanog; mESCs, mouse embryonic stem cells; mAb, monoclonal antibody; mNanog, mouse Nanog protein;
pAb, polyclonal antibody; Rb, rabbit; rhNanog, recombinant human Nanog protein; SC, Santa Cruz.