Table 2. Nanog proteins in NTERA-2 human EC cells.^* .

Ab	IP/MALDI	Tandem IP/LTQ	IP	WB	Knockdown
	(Fig. 3E-F)	(Fig. 4)	(Fig. 3A-D; Fig. S2)	(Fig. 1)	(Fig.2)
eBioscience mAb				42 kD>>35 kD>>65 kD
Kamiya Rb pAb		∼25–70 kD	42 kD	42 kD>>35 kD; 48, 65, 100 kD	42 kD>>35 kD; 48, 65, 75, 90 kD
CS Rb pAb				42 kD>>35 kD>>65 kD	42 kD>>35 kD; 65 kD
CS Rb mAb			42 kD	42 kD>>35 kD; 28,32, 70, 100 kD
SC N-17 Rb pAb				42 kD>>35 kD>55/58 kD
Biolegend Rb pAb				48 kD>35 kD>55 kD
SC H-155			42 kD	42 kD>>35 kD; 55 kD, 58 kD
R&D goat pAb	42, 60, 75, 100 kD	∼25–70 kD	42 kD	42 kD>>35 kD; 28, 65, 70 kD

*Presented are the results of various experiments using the 8 anti-Nanog Abs. The Biolegend Ab is the only Ab that does not recognize the 42 kD band as the major protein band and does not recognize the 35 kD band. Presented molecular masses are all estimated based on their migrations on SDS-PAGE.