Figure 6.

Selenite demonstrated antitumour activity in a colon cancer xenograft model by triggering apoptosis via the LEF1/CYLD/cIAPs/caspase-8 signalling pathway. (a) Fourteen days after inoculation with HCT116 cells, nude mice (seven per group) were injected with PBS or selenite (2 mg/kg/d). Tumour volumes were calculated at the indicated intervals. The data are presented as the mean±S.D. (b) Representative images and quantitative analysis of in situ labelling of apoptosis cells using TUNEL assay; original magnification, 10 × . Scale bar, 100 μm. *P<0.05. (c) Western blot analysis of proteins extracted from tumour tissues using antibodies against related molecules as indicated. (d) Immunohistochemistry results of the colon xenograft animal model using antibodies against cleaved caspase-8, cleaved caspase-3, CYLD and cIAPs (detecting both cIAP1 and cIAP2). Scale bar, 50 μm