Figure 3.

Alteration of cerebral tight junctions (TJs) decreased in the high-supplementation group of 5XFAD mice. (a) EM pictures revealed that TJs were altered in the brains of KO-Tg mice as compared with KO-WT mice. TJs of KO-Tg mice were shorter than that of KO-WT mice in the low-dose supplemented groups, and TJs of the high-dose supplemented KO-Tg mice were longer than that of standard vitamin C-treated-KO-Tg mice. n=4 for each cortex from all four groups. Arrow, TJs; RBC, red blood cells. Scale bar=2 μm. (b) Length of eight TJs from all four groups was examined and the high-dose supplemented KO-Tg mice had longer (1,225.6 nm) TJs as compared with standard vitamin C-treated-KO-Tg mice (624.2 nm). Graph shows an average of eight capillaries. *P<0.05 means the high-dose versus the low-dose supplemented KO-Tg and ^###P<0.001 means the low-dose supplemented KO-Tg versus the low-dose supplemented KO-WT mice